Regulatory Network in Protein Phosphorylation
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Kinase: JNK1(MAPK8) mitogen-activated protein kinase 8

Group: CMGC      Family: MAPK      Subfamily: JNK

Description: mitogen-activated protein kinase 8

Gene Symbol: MAPK8

Synonyms: JNK, JNK1, JNK1A2, JNK21B1/2, PRKM8, SAPK1

UniprotKB/SwissProt: MK08_HUMAN (P45983)

Function: Responds to activation by environmental stress and pro- inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. In T- cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells (By similarity). Phosphorylates heat shock factor protein 4 (HSF4). JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta- 2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.

Subcellular Localization:

Protein Domain:
PDB:1UKH

The substrate proteins of kinase JNK1(MAPK8)

No.Gene NameUniProtKB IDProtein DescriptionNumber of kinase-specific phosphorylation sitesView
1YWHAB1433B_HUMAN14-3-3 protein beta/alpha (Protein kinase C inhibitor protein 1)(KCIP-1) (Protein 1054). 1Show
2SFN1433S_HUMAN14-3-3 protein sigma (Stratifin) (Epithelial cell marker protein 1). 1Show
3YWHAZ1433Z_HUMAN14-3-3 protein zeta/delta (Protein kinase C inhibitor protein 1)(KCIP-1). 2Show
4APLP2APLP2_HUMANAmyloid-like protein 2 precursor (Amyloid protein homolog) (APPH)(CDEI box-binding protein) (CDEBP). 1Show
5ATF2ATF2_HUMANCyclic AMP-dependent transcription factor ATF-2 (Activatingtranscription factor 2) (cAMP response element-binding protein CRE-BP1) (HB16). 2Show
6ATN1ATN1_HUMANAtrophin-1 (Dentatorubral-pallidoluysian atrophy protein). 1Show
7BCL2BCL2_HUMANApoptosis regulator Bcl-2. 5Show
8BCL2L1BCLX_HUMANApoptosis regulator Bcl-X (Bcl-2-like 1 protein). 3Show
9BCL2L11BIM_HUMANBcl-2-like protein 11 (Bcl2-interacting mediator of cell death). 6Show
10CDKN1ACDN1A_HUMANCyclin-dependent kinase inhibitor 1 (p21) (CDK-interacting protein 1)(Melanoma differentiation-associated protein 6) (MDA-6). 2Show
11RCSD1CPZIP_HUMANCapz-interacting protein (Protein kinase substrate CapZIP) (RCSDdomain-containing protein 1). 3Show
12EEF2KEF2K_HUMANElongation factor 2 kinase (EC 2.7.11.20) (eEF-2 kinase) (eEF-2K)(Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase). 1Show
13ELK3ELK3_HUMANETS domain-containing protein Elk-3 (ETS-related protein NET) (ETS-related protein ERP) (SRF accessory protein 2) (SAP-2). 1Show
14FOXO4FOXO4_HUMANForkhead box protein O4 (Fork head domain transcription factor AFX1). 2Show
15NR3C1GCR_HUMANGlucocorticoid receptor (GR) (Nuclear receptor subfamily 3 group Cmember 1). 1Show
16HNRPKHNRPK_HUMANHeterogeneous nuclear ribonucleoprotein K (hnRNP K) (Transformationup-regulated nuclear protein) (TUNP). 1Show
17HSF1HSF1_HUMANHeat shock factor protein 1 (HSF 1) (Heat shock transcription factor1) (HSTF 1). 1Show
18IRS1IRS1_HUMANInsulin receptor substrate 1 (IRS-1). 2Show
19MAPK8IP1JIP1_HUMANC-jun-amino-terminal kinase-interacting protein 1 (JNK-interactingprotein 1) (JIP-1) (JNK MAP kinase scaffold protein 1) (Islet-brain 1)(IB-1) (Mitogen-activated protein kinase 8-interacting protein 1). 11Show
20MAPK8IP3JIP3_HUMANC-jun-amino-terminal kinase-interacting protein 3 (JNK-interactingprotein 3) (JIP-3) (JNK MAP kinase scaffold protein 3) (Mitogen-activated protein kinase 8-interacting protein 3). 3Show
21JUNBJUNB_HUMANTranscription factor jun-B. 2Show
22JUNJUN_HUMANTranscription factor AP-1 (Activator protein 1) (AP1) (Proto-oncogenec-jun) (V-jun avian sarcoma virus 17 oncogene homolog) (p39). 6Show
23KRT8K2C8_HUMANKeratin, type II cytoskeletal 8 (Cytokeratin-8) (CK-8) (Keratin-8)(K8). 2Show
24MYCMYC_HUMANMyc proto-oncogene protein (c-Myc) (Transcription factor p64). 2Show
25TP53P53_HUMANCellular tumor antigen p53 (Tumor suppressor p53) (Phosphoprotein p53)(Antigen NY-CO-13). 1Show
26PXNPAXI_HUMANPaxillin. 1Show
27PPARGPPARG_HUMANPeroxisome proliferator-activated receptor gamma (PPAR-gamma) (Nuclearreceptor subfamily 1 group C member 3). 2Show
28STAT3STAT3_HUMANSignal transducer and activator of transcription 3 (Acute-phaseresponse factor). 2Show



The substrate proteins of kinase family JNK

No.Gene NameUniProtKB IDProtein DescriptionNumber of kinase family-specific phosphorylated sitesView
1YWHAB1433B_HUMAN14-3-3 protein beta/alpha (Protein kinase C inhibitor protein 1)(KCIP-1) (Protein 1054). 1Show
2SFN1433S_HUMAN14-3-3 protein sigma (Stratifin) (Epithelial cell marker protein 1). 1Show
3YWHAZ1433Z_HUMAN14-3-3 protein zeta/delta (Protein kinase C inhibitor protein 1)(KCIP-1). 2Show
4SH3BP53BP5_HUMANSH3 domain-binding protein 5 (SH3 domain-binding protein thatpreferentially associates with BTK). 4Show
5APPA4_HUMANAmyloid beta A4 protein precursor (APP) (ABPP) (Alzheimer diseaseamyloid protein) (Cerebral vascular amyloid peptide) (CVAP) (Proteasenexin-II) (PN-II) (APPI) (PreA4) [Contains: Soluble APP-alpha (S-APP-alpha); Soluble APP-beta (S-APP-beta); C99; Beta-amyloid protein 42(Beta-APP42); Beta-amyloid protein 40 (Beta-APP40); C83; P3(42);P3(40); Gamma-CTF(59) (Gamma-secretase C-terminal fragment 59)(Amyloid intracellular domain 59) (AID(59)) (AICD-59); Gamma-CTF(57)(Gamma-secretase C-terminal fragment 57) (Amyloid intracellular domain57) (AID(57)) (AICD-57); Gamma-CTF(50) (Gamma-secretase C-terminalfragment 50) (Amyloid intracellular domain 50) (AID(50)) (AICD-50);C31]. 1Show
6APLP2APLP2_HUMANAmyloid-like protein 2 precursor (Amyloid protein homolog) (APPH)(CDEI box-binding protein) (CDEBP). 1Show
7ATF2ATF2_HUMANCyclic AMP-dependent transcription factor ATF-2 (Activatingtranscription factor 2) (cAMP response element-binding protein CRE-BP1) (HB16). 4Show
8ATN1ATN1_HUMANAtrophin-1 (Dentatorubral-pallidoluysian atrophy protein). 1Show
9BCL2BCL2_HUMANApoptosis regulator Bcl-2. 5Show
10BCL2L1BCLX_HUMANApoptosis regulator Bcl-X (Bcl-2-like 1 protein). 3Show
11BCL2L11BIM_HUMANBcl-2-like protein 11 (Bcl2-interacting mediator of cell death). 15Show
12CDKN1ACDN1A_HUMANCyclin-dependent kinase inhibitor 1 (p21) (CDK-interacting protein 1)(Melanoma differentiation-associated protein 6) (MDA-6). 2Show
13RCSD1CPZIP_HUMANCapz-interacting protein (Protein kinase substrate CapZIP) (RCSDdomain-containing protein 1). 3Show
14EEF2KEF2K_HUMANElongation factor 2 kinase (EC 2.7.11.20) (eEF-2 kinase) (eEF-2K)(Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase). 2Show
15ELK1ELK1_HUMANETS domain-containing protein Elk-1. 4Show
16ELK3ELK3_HUMANETS domain-containing protein Elk-3 (ETS-related protein NET) (ETS-related protein ERP) (SRF accessory protein 2) (SAP-2). 3Show
17FOXO4FOXO4_HUMANForkhead box protein O4 (Fork head domain transcription factor AFX1). 2Show
18NR3C1GCR_HUMANGlucocorticoid receptor (GR) (Nuclear receptor subfamily 3 group Cmember 1). 2Show
19H3F3AH33_HUMANHistone H3.3. 1Show
20HNRPKHNRPK_HUMANHeterogeneous nuclear ribonucleoprotein K (hnRNP K) (Transformationup-regulated nuclear protein) (TUNP). 5Show
21HSF1HSF1_HUMANHeat shock factor protein 1 (HSF 1) (Heat shock transcription factor1) (HSTF 1). 2Show
22IRS1IRS1_HUMANInsulin receptor substrate 1 (IRS-1). 2Show
23MAPK8IP1JIP1_HUMANC-jun-amino-terminal kinase-interacting protein 1 (JNK-interactingprotein 1) (JIP-1) (JNK MAP kinase scaffold protein 1) (Islet-brain 1)(IB-1) (Mitogen-activated protein kinase 8-interacting protein 1). 18Show
24MAPK8IP3JIP3_HUMANC-jun-amino-terminal kinase-interacting protein 3 (JNK-interactingprotein 3) (JIP-3) (JNK MAP kinase scaffold protein 3) (Mitogen-activated protein kinase 8-interacting protein 3). 6Show
25JUNBJUNB_HUMANTranscription factor jun-B. 2Show
26JUNJUN_HUMANTranscription factor AP-1 (Activator protein 1) (AP1) (Proto-oncogenec-jun) (V-jun avian sarcoma virus 17 oncogene homolog) (p39). 6Show
27KRT8K2C8_HUMANKeratin, type II cytoskeletal 8 (Cytokeratin-8) (CK-8) (Keratin-8)(K8). 2Show
28MAPKAPK5MAPK5_HUMANMAP kinase-activated protein kinase 5 (EC 2.7.11.1) (MAPK-activatedprotein kinase 5) (MAPKAP kinase 5) (p38-regulated/activated proteinkinase). 1Show
29CDC25CMPIP3_HUMANM-phase inducer phosphatase 3 (EC 3.1.3.48) (Dual specificityphosphatase Cdc25C). 1Show
30MYCMYC_HUMANMyc proto-oncogene protein (c-Myc) (Transcription factor p64). 2Show
31NFATC3NFAC3_HUMANNuclear factor of activated T-cells, cytoplasmic 3 (NF-ATc3) (NFATc3)(T-cell transcription factor NFAT4) (NF-AT4) (NFATx). 4Show
32TP53P53_HUMANCellular tumor antigen p53 (Tumor suppressor p53) (Phosphoprotein p53)(Antigen NY-CO-13). 1Show
33PXNPAXI_HUMANPaxillin. 1Show
34PPARGPPARG_HUMANPeroxisome proliferator-activated receptor gamma (PPAR-gamma) (Nuclearreceptor subfamily 1 group C member 3). 2Show
35jdp2Q8WYK2_HUMANJun dimerization protein 2 (Jun dimerization protein 2, isoformCRA_a). 1Show
36RRN3RRN3_HUMANRNA polymerase I-specific transcription initiation factor RRN3(Transcription initiation factor IA) (TIF-IA). 1Show
37STAT3STAT3_HUMANSignal transducer and activator of transcription 3 (Acute-phaseresponse factor). 2Show