Regulatory Network in Protein Phosphorylation
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Substrate: PTEN

Description: Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN (EC (EC (Phosphatase and tensin homolog) (Mutated in multipleadvanced cancers 1).

Synonyms: MMAC1, TEP1

Ensembl ID: ENSG00000171862

UniprotKB/SwissProt: PTEN_HUMAN (P60484)

Function: Tumor suppressor. Acts as a dual-specificity protein phosphatase, dephosphorylating tyrosine-, serine- and threonine- phosphorylated proteins. Also acts as a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring from phosphatidylinositol 3,4,5-trisphosphate, phosphatidylinositol 3,4-diphosphate, phosphatidylinositol 3- phosphate and inositol 1,3,4,5-tetrakisphosphate with order of substrate preference in vitro PtdIns(3,4,5)P3 > PtdIns(3,4)P2 > PtdIns3P > Ins(1,3,4,5)P4. The lipid phosphatase activity is critical for its tumor suppressor function. Antagonizes the PI3K- AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival. The unphosphorylated form cooperates with AIP1 to suppress AKT1 activation. Dephosphorylates tyrosine-phosphorylated focal adhesion kinase and inhibits cell migration and integrin-mediated cell spreading and focal adhesion formation. May be a negative regulator of insulin signaling and glucose metabolism in adipose tissue.

Other Modifications: View all modification sites in dbPTM

Protein Subcellular Localization: Cytoplasm.
Protein Domain and Phosphorylation Sites:

The phosphorylated sites of PTEN

No.SubstrateUniProtKB IDPositionPhosphoPeptideSolvent AccessibilityCatalytic kinaseSourceComputational Annotation of Catalytic KinaseInteracting PartnersExpression Analysis
1PTENPTEN_HUMANS229IYSSN S GPTRR 48.36%ROCK1 Phospho.ELM 7.0  ViewAnalyzing
2PTENPTEN_HUMANT232SNSGP T RREDK 45.97%ROCK1 Phospho.ELM 7.0  ViewAnalyzing
3PTENPTEN_HUMANY240EDKFM Y FEFPQ 11.77% HPRD:03431(in vitro)View   
4PTENPTEN_HUMANY240EDKFM Y FEFPQ 11.77%Lck Phospho.ELM 7.0  ViewAnalyzing
5PTENPTEN_HUMANY315DNDKE Y LVLTL 14.09% HPRD:03431(in vitro)View   
6PTENPTEN_HUMANY315DNDKE Y LVLTL 14.09%Lck Phospho.ELM 7.0  ViewAnalyzing
7PTENPTEN_HUMANS362PEASS S TSVTP 23.39%GSK3B(GSK3 beta) Phospho.ELM 7.0 ViewAnalyzing
8PTENPTEN_HUMANT366SSTSV T PDVSD 17.34%CK2a1(CK2 alpha) Phospho.ELM 7.0 ViewAnalyzing
9PTENPTEN_HUMANT366SSTSV T PDVSD 17.34%CK2a1 HPRD:03431(in vitro;in vivo)  ViewAnalyzing
10PTENPTEN_HUMANT366SSTSV T PDVSD 17.34%CK2_group Phospho.ELM 7.0 ViewAnalyzing
11PTENPTEN_HUMANT366SSTSV T PDVSD 17.34% Swiss-Prot 55.0 View   
12PTENPTEN_HUMANT366SSTSV T PDVSD 17.34%GSK3B(GSK3 beta) Phospho.ELM 7.0 ViewAnalyzing
13PTENPTEN_HUMANT366SSTSV T PDVSD 17.34%CK2a2 HPRD:03431(in vitro;in vivo)  ViewAnalyzing
14PTENPTEN_HUMANT366SSTSV T PDVSD 17.34%CK2a1-rs HPRD:03431(in vitro;in vivo)  ViewAnalyzing
15PTENPTEN_HUMANS370VTPDV S DNEPD 41.94%CK2_group Swiss-Prot 55.0  ViewAnalyzing
16PTENPTEN_HUMANS370VTPDV S DNEPD 41.94%CK2_group Phospho.ELM 7.0 ViewAnalyzing
17PTENPTEN_HUMANS370VTPDV S DNEPD 41.94%CK2a1-rs HPRD:03431(in vitro;in vivo)  ViewAnalyzing
18PTENPTEN_HUMANS370VTPDV S DNEPD 41.94%CK2a1 HPRD:03431(in vitro;in vivo)  ViewAnalyzing
19PTENPTEN_HUMANS370VTPDV S DNEPD 41.94%CK2a2 HPRD:03431(in vitro;in vivo)  ViewAnalyzing
20PTENPTEN_HUMANS370VTPDV S DNEPD 41.94%CK2a1(CK2 alpha) Phospho.ELM 7.0 ViewAnalyzing
21PTENPTEN_HUMANS380DHYRY S DTTDS 21.34%AKT1 HPRD:03431(in vitro;in vivo)  ViewAnalyzing
22PTENPTEN_HUMANS380DHYRY S DTTDS 21.34%CK2a1(CK2 alpha) Phospho.ELM 7.0 ViewAnalyzing
23PTENPTEN_HUMANS380DHYRY S DTTDS 21.34%AKT_group Phospho.ELM 7.0 ViewAnalyzing
24PTENPTEN_HUMANT382YRYSD T TDSDP 26.94%CK2a1(CK2 alpha) Phospho.ELM 7.0 ViewAnalyzing
25PTENPTEN_HUMANT382YRYSD T TDSDP 26.94% HPRD:03431(in vitro)View   
26PTENPTEN_HUMANT383RYSDT T DSDPE 38.20% Phospho.ELM 7.0View   
27PTENPTEN_HUMANT383RYSDT T DSDPE 38.20% HPRD:03431(in vitro)View   
28PTENPTEN_HUMANS385SDTTD S DPENE 53.63%AKT_group Phospho.ELM 7.0 ViewAnalyzing
29PTENPTEN_HUMANS385SDTTD S DPENE 53.63%CK2_group Phospho.ELM 7.0 ViewAnalyzing
30PTENPTEN_HUMANS385SDTTD S DPENE 53.63%AKT1 HPRD:03431(in vitro;in vivo)  ViewAnalyzing
31PTENPTEN_HUMANS385SDTTD S DPENE 53.63%LKB1 HPRD:03431(in vitro)  ViewAnalyzing
32PTENPTEN_HUMANS385SDTTD S DPENE 53.63%CK2_group Swiss-Prot 55.0  ViewAnalyzing
33PTENPTEN_HUMANS385SDTTD S DPENE 53.63%CK2a1(CK2 alpha) Phospho.ELM 7.0 ViewAnalyzing
34PTENPTEN_HUMANT401QHTQI T KV 18.88% Swiss-Prot 55.0 View