Regulatory Network in Protein Phosphorylation
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Substrate: PAM

Description: Peptidyl-glycine alpha-amidating monooxygenase precursor (PAM)[Includes: Peptidylglycine alpha-hydroxylating monooxygenase(EC 1.14.17.3) (PHM); Peptidyl-alpha-hydroxyglycine alpha-amidatinglyase (EC 4.3.2.5) (Peptidylamidoglycolate lyase) (PAL)].

Ensembl ID: ENSG00000145730

UniprotKB/SwissProt: AMD_HUMAN (P19021)

Function: Bifunctional enzyme that catalyzes 2 sequencial steps in C-terminal alpha-amidation of peptides. The monooxygenase part produces an unstable peptidyl(2-hydroxyglycine) intermediate that is dismutated to glyoxylate and the corresponding desglycine peptide amide by the lyase part. C-terminal amidation of peptides such as neuropeptides is essential for full biological activity.

Other Modifications: View all modification sites in dbPTM

Protein Subcellular Localization: Isoform 1: Membrane; Single-pass type I membrane protein. Isoform 2: Membrane; Single-pass type I membrane protein. Isoform 3: Secreted. Note=Secreted from secretory granules. Isoform 4: Secreted. Note=Secreted from secretory granules.
Protein Domain and Phosphorylation Sites:

The phosphorylated sites of PAM

No.SubstrateUniProtKB IDPositionPhosphoPeptideSolvent AccessibilityCatalytic kinaseSourceComputational Annotation of Catalytic KinaseInteracting PartnersExpression Analysis
1PAMAMD_HUMANS918FRGKG S GGLNL 32.92% Swiss-Prot 55.0 (Similarity)View   
2PAMAMD_HUMANS929GNFFA S RKGYS 29.39%PKC_group Phospho.ELM 7.0 ViewAnalyzing
3PAMAMD_HUMANS929GNFFA S RKGYS 29.39%PKCa HPRD:01361(in vitro)  ViewAnalyzing
4PAMAMD_HUMANS934SRKGY S RKGFD 35.86%PKCa HPRD:01361(in vitro)  ViewAnalyzing
5PAMAMD_HUMANS934SRKGY S RKGFD 35.86%PKC_group Phospho.ELM 7.0 ViewAnalyzing
6PAMAMD_HUMANS942GFDRL S TEGSD 25.88% Phospho.ELM 7.0View   
7PAMAMD_HUMANS946LSTEG S DQEKE 29.86%P-CIP2 Phospho.ELM 7.0 ViewAnalyzing
8PAMAMD_HUMANS946LSTEG S DQEKE 29.86%KIS HPRD:01361(in vitro)  ViewAnalyzing
9PAMAMD_HUMANS955KEDDG S ESEEE 44.97% Phospho.ELM 7.0View   
10PAMAMD_HUMANS955KEDDG S ESEEE 44.97% Swiss-Prot 55.0 View   
11PAMAMD_HUMANS957DDGSE S EEEYS 52.59% Phospho.ELM 7.0View   
12PAMAMD_HUMANS957DDGSE S EEEYS 52.59% Swiss-Prot 55.0 View