Regulatory Network in Protein Phosphorylation
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Substrate: WEE1

Description: Wee1-like protein kinase (EC (Wee1A kinase) (WEE1hu).

Ensembl ID: ENSG00000166483

UniprotKB/SwissProt: WEE1_HUMAN (P30291)

Function: May act as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDC2 before the onset of mitosis. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation. Specifically phosphorylates and inactivates cyclin B1-complexed CDC2 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDC2 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDC2 does not occur.

Other Modifications: View all modification sites in dbPTM

Protein Subcellular Localization: Nucleus.
Protein Domain and Phosphorylation Sites:

The phosphorylated sites of WEE1

No.SubstrateUniProtKB IDPositionPhosphoPeptideSolvent AccessibilityCatalytic kinaseSourceComputational Annotation of Catalytic KinaseInteracting PartnersExpression Analysis
1WEE1WEE1_HUMANS53STGED S AFQEP 27.04%PLK1 Phospho.ELM 7.0  ViewAnalyzing
2WEE1WEE1_HUMANS53STGED S AFQEP 27.04%PLK1 HPRD:01907(in vitro)  ViewAnalyzing
3WEE1WEE1_HUMANS121EEGFG S SSPVK 25.94% Phospho.ELM 7.0View   
4WEE1WEE1_HUMANS123GFGSS S PVKSP 35.10% HPRD:01907(in vitro)View   
5WEE1WEE1_HUMANS123GFGSS S PVKSP 35.10%CDC2 Swiss-Prot 55.0 (Potential)  ViewAnalyzing
6WEE1WEE1_HUMANS123GFGSS S PVKSP 35.10%CDC2(CDK1) Phospho.ELM 7.0 ViewAnalyzing
7WEE1WEE1_HUMANS127SSPVK S PAAPY 21.72% Swiss-Prot 55.0 View   
8WEE1WEE1_HUMANS127SSPVK S PAAPY 21.72% Phospho.ELM 7.0View   
9WEE1WEE1_HUMANS139LGSSF S PVRCG 25.16% HPRD:01907(in vivo)View   
10WEE1WEE1_HUMANS139LGSSF S PVRCG 25.16% Phospho.ELM 7.0View   
11WEE1WEE1_HUMANS139LGSSF S PVRCG 25.16% Swiss-Prot 55.0 View   
12WEE1WEE1_HUMANS165GEGRR S PRPDH 24.73% Phospho.ELM 7.0View   
13WEE1WEE1_HUMANS165GEGRR S PRPDH 24.73% Swiss-Prot 55.0 View   
14WEE1WEE1_HUMANT173PDHPG T PPHKT 37.79% Swiss-Prot 55.0 View   
15WEE1WEE1_HUMANT173PDHPG T PPHKT 37.79% Phospho.ELM 7.0View   
16WEE1WEE1_HUMANS211VKLRG S SLFMD 19.89% HPRD:01907(in vitro)View   
17WEE1WEE1_HUMANS212KLRGS S LFMDT 28.57% HPRD:01907(in vitro)View   
18WEE1WEE1_HUMANS559PERRP S AMALV 28.94% HPRD:01907(in vitro)View   
19WEE1WEE1_HUMANS642MNRSV S LTIY 22.50%BRSK1 HPRD:01907(in vitro)  ViewAnalyzing
20WEE1WEE1_HUMANS642MNRSV S LTIY 22.50%BRSK2 HPRD:01907(in vivo)  ViewAnalyzing
21WEE1WEE1_HUMANS642MNRSV S LTIY 22.50%BRSK1 Swiss-Prot 55.0 (in vitro)  ViewAnalyzing
22WEE1WEE1_HUMANS642MNRSV S LTIY 22.50%AKT_group Phospho.ELM 7.0 ViewAnalyzing