Regulatory Network in Protein Phosphorylation
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Substrate: CHEK2

Description: Serine/threonine-protein kinase Chk2 (EC 2.7.11.1) (Cds1).

Synonyms: CHK2, RAD53

Ensembl ID: ENSG00000183765

UniprotKB/SwissProt: CHK2_HUMAN (O96017)

Function: Regulates cell cycle checkpoints and apoptosis in response to DNA damage, particularly to DNA double-strand breaks. Inhibits CDC25C phosphatase by phosphorylation on 'Ser-216', preventing the entry into mitosis. May also play a role in meiosis. Regulates the TP53 tumor suppressor through phosphorylation at 'Thr-18' and 'Ser-20'.

Other Modifications: View all modification sites in dbPTM

Protein Subcellular Localization: Isoform 2: Nucleus. Note=Isoform 10 is present throughout the cell. Isoform 4: Nucleus. Isoform 7: Nucleus. Isoform 9: Nucleus. Isoform 12: Nucleus.
PDB:1GXC
Protein Domain and Phosphorylation Sites:

The phosphorylated sites of CHEK2

No.SubstrateUniProtKB IDPositionPhosphoPeptideSolvent AccessibilityCatalytic kinaseSourceComputational Annotation of Catalytic KinaseInteracting PartnersExpression Analysis
1CHEK2CHK2_HUMANS19GSSAC S QPHGS 46.60%ATM HPRD:05084(in vitro)  ViewAnalyzing
2CHEK2CHK2_HUMANS19GSSAC S QPHGS 46.60%ATM Phospho.ELM 7.0  ViewAnalyzing
3CHEK2CHK2_HUMANS19GSSAC S QPHGS 46.60%ATR HPRD:05084(in vitro)  ViewAnalyzing
4CHEK2CHK2_HUMANT26PHGSV T QSQGS 24.97%ATM Phospho.ELM 7.0  ViewAnalyzing
5CHEK2CHK2_HUMANT26PHGSV T QSQGS 24.97%ATR HPRD:05084(in vitro)  ViewAnalyzing
6CHEK2CHK2_HUMANT26PHGSV T QSQGS 24.97%PLK1 HPRD:05084(in vitro)  ViewAnalyzing
7CHEK2CHK2_HUMANT26PHGSV T QSQGS 24.97%PLK1 Phospho.ELM 7.0  ViewAnalyzing
8CHEK2CHK2_HUMANT26PHGSV T QSQGS 24.97%ATM HPRD:05084(in vitro;in vivo)  ViewAnalyzing
9CHEK2CHK2_HUMANS28GSVTQ S QGSSS 27.43%ATM Phospho.ELM 7.0  ViewAnalyzing
10CHEK2CHK2_HUMANS28GSVTQ S QGSSS 27.43%ATM HPRD:05084(in vitro;in vivo)  ViewAnalyzing
11CHEK2CHK2_HUMANS28GSVTQ S QGSSS 27.43%ATR HPRD:05084(in vitro)  ViewAnalyzing
12CHEK2CHK2_HUMANS33SQGSS S QSQGI 35.23%ATM HPRD:05084(in vitro)  ViewAnalyzing
13CHEK2CHK2_HUMANS33SQGSS S QSQGI 35.23%ATM Phospho.ELM 7.0  ViewAnalyzing
14CHEK2CHK2_HUMANS33SQGSS S QSQGI 35.23%ATR HPRD:05084(in vitro)  ViewAnalyzing
15CHEK2CHK2_HUMANS35GSSSQ S QGISS 32.11%ATR HPRD:05084(in vitro)  ViewAnalyzing
16CHEK2CHK2_HUMANS35GSSSQ S QGISS 32.11%ATM HPRD:05084(in vitro)  ViewAnalyzing
17CHEK2CHK2_HUMANS35GSSSQ S QGISS 32.11%ATM Phospho.ELM 7.0  ViewAnalyzing
18CHEK2CHK2_HUMANS50TMPNS S QSSHS 40.50%ATM Phospho.ELM 7.0  ViewAnalyzing
19CHEK2CHK2_HUMANS50TMPNS S QSSHS 40.50%ATM HPRD:05084(in vitro)  ViewAnalyzing
20CHEK2CHK2_HUMANS50TMPNS S QSSHS 40.50%ATR HPRD:05084(in vitro)  ViewAnalyzing
21CHEK2CHK2_HUMANT68LETVS T QELYS 23.02%ATM HPRD:05084(in vitro;in vivo)  ViewAnalyzing
22CHEK2CHK2_HUMANT68LETVS T QELYS 23.02%ATM HPRD:05084(in vitro;in vivo)  ViewAnalyzing
23CHEK2CHK2_HUMANT68LETVS T QELYS 23.02%ATM HPRD:05084(in vivo)  ViewAnalyzing
24CHEK2CHK2_HUMANT68LETVS T QELYS 23.02%ATM Phospho.ELM 7.0  ViewAnalyzing
25CHEK2CHK2_HUMANT68LETVS T QELYS 23.02%PLK1 Phospho.ELM 7.0  ViewAnalyzing
26CHEK2CHK2_HUMANT68LETVS T QELYS 23.02%PLK1 HPRD:05084(in vitro;in vivo)  ViewAnalyzing
27CHEK2CHK2_HUMANT68LETVS T QELYS 23.02%PLK3 HPRD:05084(in vitro;in vivo)  ViewAnalyzing
28CHEK2CHK2_HUMANT68LETVS T QELYS 23.02%CHK2 Phospho.ELM 7.0  ViewAnalyzing
29CHEK2CHK2_HUMANT68LETVS T QELYS 23.02%CHK2 HPRD:05084(in vitro;in vivo)  ViewAnalyzing
30CHEK2CHK2_HUMANT68LETVS T QELYS 23.02%ZAK Swiss-Prot 55.0   ViewAnalyzing
31CHEK2CHK2_HUMANT383TSLMR T LCGTP 16.66%CHK2 Phospho.ELM 7.0  ViewAnalyzing
32CHEK2CHK2_HUMANT383TSLMR T LCGTP 16.66%CHK2 HPRD:05084(in vitro;in vivo)  ViewAnalyzing
33CHEK2CHK2_HUMANT387RTLCG T PTYLA 16.09%CHK2 HPRD:05084(in vitro;in vivo)  ViewAnalyzing
34CHEK2CHK2_HUMANT387RTLCG T PTYLA 16.09%CHK2 Phospho.ELM 7.0  ViewAnalyzing
35CHEK2CHK2_HUMANS516VLAQP S TSRKR 27.25%CHK2 HPRD:05084(in vitro;in vivo)  ViewAnalyzing
36CHEK2CHK2_HUMANS516VLAQP S TSRKR 27.25%CHK2 Phospho.ELM 7.0  ViewAnalyzing